Temporal Resolution & Sensitivity

Only CellOPTIQ® is able to determine the critical type of EADs present and report on their classification.


Voltage Assay features include:

Proprietary Technology

Clyde’s voltage assay uses proprietary recording techniques and bespoke software to measure transmembrane voltage

Rapid Sensing

Clyde uses a non-toxic ratiometric dye to enable rapid (<1ms) sensing of changes in voltage

Ultra Sensitivity

CellOPTIQ® can record data at >10 kHz, giving it the ability to detect subtle changes in upstroke kinetics

CiPA Standards

CiPA standardised measurements of action potential

Detailed Analysis

Producing detailed analysis of all action potential phases: upstroke, early-, middle- and late-stage repolarisation

Repeat for up to 20 days

Able to conduct chronic studies with repeated measurements up to 20 days

2D & 3D

Works with 2D or 3D structures

Serum Free

Compounds tested in serum free media avoiding variation of results due to serum



Typical Voltage Data - Cisapride

Below is the output from CellOPTIQ for the action of Cisapride on spontaneously beating hiPSC cardiomyocytes. The data shows a typical response to Cisapride with the prolonging of APD50 and APD90 causing triangulation. Prolongation of ADP90 starts at a concentration, very similar to that seen in humans (QTc). The effects are seen at concentrations within 10 fold of Cmax, therefore Cisapride is designated medium risk of pro arrhythmia.


Assessment of a compound's potential to cause EADs is of vital importance in determining cardiotoxic potential. Older technologies can detect EADs, but only in a presence/absence model. Only CLYDE is able to determine the critical type of EAD present and report on their classification.



EAD Classification

Examples of EADs Elucidated on CellOPTIQ®

Examples of EADs Elucidated on Clydes’ CellOPTIQ platform

Start a study with Clyde

To enquire about how Clyde could help with your project complete the enquiry form below and we’ll be get back to you as soon as we can. Alternatively email us at info@clydebio.com