Intracellular calcium transient measurements are critical for the understanding of cardiac function. Clyde’s CellOPTIQ® is a powerful tool, offering more accuracy and higher throughput than other assays.
Clyde’s Calcium Assay:
The Rational Approach
- Clyde Biosciences’ CellOPTIQ® system uses ratiometric dyes to measure calcium
- By analysing the ratio between the two wavelengths CellOPTIQ® can obtain measurements which are unaffected by movement artefact or variations in cell layer thickness
- This yields data that is more accurate, with significantly reduced signal-to-noise
CellOPTIQ® can measure simultaneous voltage and calcium at 10,000 times per second providing detailed information about their interaction in arrhythmic action potentials where e.g. EADs are present, which sequential measurement cannot do.
Example Data - Verapamil
Verapamil was used as an example of mixed action compound. Low concentrations of Verapamil (30nM), an antiarrhythmic drug with known mixed hERG and L-type Ca2+ channel activity, had no effect on APD (APD75 = 1.5 ± 0.7%) (Panel A), whereas the amplitude of Ca2+ transient was significantly reduced (45 ± 8%, p<0.05) (Panel B). This further highlights the need for measuring more than one parameter when testing compounds with mixed ion channel activity.