Temporal Resolution & Sensitivity
Only CellOPTIQ® is able to determine the critical type of EADs present and report on their classification.
Voltage Assay features include:
Proprietary Technology
Clyde’s voltage assay uses proprietary recording techniques and bespoke software to measure transmembrane voltageRapid Sensing
Clyde uses a non-toxic ratiometric dye to enable rapid (<1ms) sensing of changes in voltageUltra Sensitivity
CellOPTIQ® can record data at >10 kHz, giving it the ability to detect subtle changes in upstroke kineticsCiPA Standards
CiPA standardised measurements of action potentialDetailed Analysis
Producing detailed analysis of all action potential phases: upstroke, early-, middle- and late-stage repolarisationRepeat for up to 20 days
Able to conduct chronic studies with repeated measurements up to 20 days2D & 3D
Works with 2D or 3D structuresSerum Free
Compounds tested in serum free media avoiding variation of results due to serumTypical Voltage Data - Cisapride
Below is the output from CellOPTIQ for the action of Cisapride on spontaneously beating hiPSC cardiomyocytes. The data shows a typical response to Cisapride with the prolonging of APD50 and APD90 causing triangulation. Prolongation of ADP90 starts at a concentration, very similar to that seen in humans (QTc). The effects are seen at concentrations within 10 fold of Cmax, therefore Cisapride is designated medium risk of pro arrhythmia.
Assessment of a compound's potential to cause EADs is of vital importance in determining cardiotoxic potential. Older technologies can detect EADs, but only in a presence/absence model. Only CLYDE is able to determine the critical type of EAD present and report on their classification.
EAD Classification
Examples of EADs Elucidated on CellOPTIQ®
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